Document Type : Review Paper


Department of Pharmaceutics, College of Pharmacy, University of Baghdad, Baghdad, Iraq


Background: The use of conventional animal testing to determine how medications affect a biological system comes with many challenges. In recent years, a variety of techniques to achieve these goals were presented, one of these is cell line creation technology that has dramatically increased in usefulness and efficiency of drug discovery researches. Cell culture is the term used to describe the removal of cells from an animal or plant and their subsequent cultivation in a lab setting. The Caco-2 model is commonly used in the early stages of drug discovery to make decisions about the permeability and/or absorption of tested chemicals. It is obvious that novel 3D cell culture models offer enormous potential for disease modeling and medication efficacy and safety assessment. However, the challenges provided by the inherent traits of Caco-2 cell variants and inter-laboratory methods have led to the development of irreducible data. Conclusion: These restrictions have an impact on the extrapolation of preclinical research results to clinical investigations on drug-drug and herbal-drug interactions.


Main Subjects

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