Iraqi Journal of Pharmacy

Abstract

Objective: A reverse phase high performance liquid chromatographic method (RP-HPLC) has been developed for the determination of  tadalafil in the pharmaceutical formulations  and environmental wastewater samples.
Method: Different analytical columns with various  stationary phases were tested. Good separation was achieved using supelcoC18 column (25cm x 4.6 mm. 5 µm) . The mobile phase (methanol: water: Triethylamine) , pH adjusted to 4.0 with dilute phosphoric acid was pumped at a flow rate of 1.3 ml/min in the ratio of 60:38:2   and the peaks  were detected at 220 nm.
Results: Linearity was obtained in the concentration range of 0.0 4-0.28 mg/ml .The RSD was found to be less than 1% indicating the method is precise and the recovery was100±1.25% indicating the method is accurate
 Conclusion: The proposed HPLC method may be used for determining tadalafil in pure drug samples , pharmaceutical dosage forms and environmental wastewater samples.