Pharmacognosy & Natural Products
Dilbreen H. Abdulqader; Sami R. AL-Zubaydi; Enas Jawad Kadhim
Abstract
Background: Malvaceae is a large herbal family with more than a hundred genera and thousands of species. Some of these species are well-known for their therapeutic activity, including Alcea. Aim: The purpose of this study is to isolate and identify the biologically and therapeutically active constituents ...
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Background: Malvaceae is a large herbal family with more than a hundred genera and thousands of species. Some of these species are well-known for their therapeutic activity, including Alcea. Aim: The purpose of this study is to isolate and identify the biologically and therapeutically active constituents of the flowers and leaves of Alcea kurdica alongside the characterization of the extract composition. Methods: Extraction was done with 70% ethanol using Soxhlet extraction method. The fractionated extracts were condensed, dried, and labeled as (LEA) for organic leaf extract, (LW) for aqueous leaf extract, (FEA) for organic flower extract, and (FW) for aqueous flower extract using ethyl acetate with an equal volume of water and shaken three times. Results: The isolated flavonoids and sterols were identified by comparison with the chromatograms of the standard compounds, which were prepared under standard circumstances. Flavonoids and sterols were found in the leaves and flower extract of Alcea kurdica after HPLC analysis.
Zena Sattam Hamad; Basil Mohammed Yahya
Abstract
Objectives: to evaluate a rapid and sensitive high performance liquid chromatographic method for the determination of metoclopramide in rat serum.
Methods: The assay was performed after liquid-liquid extraction with sodium hydroxide and dichloromethane.
Results and conclusion: Chromatographic separations ...
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Objectives: to evaluate a rapid and sensitive high performance liquid chromatographic method for the determination of metoclopramide in rat serum.
Methods: The assay was performed after liquid-liquid extraction with sodium hydroxide and dichloromethane.
Results and conclusion: Chromatographic separations were performed on C18 stationary phase with a mobile phase composed of acetonitrile : 1% triethyleamine (50:50,v/v) at pH (6.8). Analytes were detected at wave length of 270nm. This method was validated for specificity and linearity with a correlation coefficient, r=0.94.
Zena Sattam Hamad; Basil Mohammed Yahya
Abstract
Objective: A method for simultaneous determination of chlordiazepoxide in rat plasma using liquid-liquid extraction followed by high performance liquid chromatography (HPLC) is described.
Methods: The analytes were separated employing a LC-18 column (250mm×4.6mm,5µm) at ambient temperature using ...
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Objective: A method for simultaneous determination of chlordiazepoxide in rat plasma using liquid-liquid extraction followed by high performance liquid chromatography (HPLC) is described.
Methods: The analytes were separated employing a LC-18 column (250mm×4.6mm,5µm) at ambient temperature using methanol and water (60 : 40 v/v) as a mobile phase at a flow rate 1.2 ml/min. Ultra violet (UV) detection was carried out at 254 nm. Employing liquid-liquid extraction (LLE), the best conditions were achieved with the extraction of 0.5 ml plasma using 7.5 ml deionized water, 0.5ml of 0.1M NaOH and 2.5 ml diethyl ether, the mixture was shaken for 15 min, centrifuged, and an aliquot of the ether phase evaporated off in a water bath at 30 °C. The residue was reconstituted with the mobile phase 50 µl followed by HPLC analysis.
Results and conclution: This method was validated for specificity and linearity with excellent correlation coefficient (r=0.99) showed their suitable applicability in order to examine chlordiazepoxide in rat plasma.